Expression of 5HT7 Receptor in rat dorsal raphe nucleus (DRN) - Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-5HT7 Receptor/HTR7 (extracellular) Antibody (#ASR-037), (1:300), followed by goat anti-rabbit-AlexaFluor-488. A. 5HT7R immunoreactivity (green) appears in neuronal profiles in the lateral DRN (arrows). B. Pre-incubation of the antibody with 5HT7 Receptor/HTR7 (extracellular) Blocking Peptide (#BLP-SR037), suppresses staining. Cell nuclei are stained with DAPI (blue). LDRN = lateral DRN, MDRN = medial DRN.

Peptide CGEQINYGRVEK, corresponding to amino acid residues 73 - 84 of rat 5-HT7 (Accession P32305). Extracellular, N-terminus

Western blot analysis of rat brain lysate (lanes 1 and 3) and mouse brain lysate (lanes 2 and 4): - 1, 2. Anti-5HT7 Receptor/HTR7 (extracellular) Antibody (#ASR-037), (1:400). 3, 4. Anti-5HT7 Receptor/HTR7 (extracellular) Antibody, preincubated with 5HT7 Receptor/HTR7 (extracellular) Blocking Peptide (#BLP-SR037).